Study on Extraction Process of Total Flavonoids from Astragalus by Ultraviolet Spectrophotometry Keywords: Astragalus; Flavonoids; Extraction; Ultraviolet Spectrophotometry;
Aesthetic instrument
; UV-1500PC; Abstract: Flavonoids generally refer to a series of compounds in which two benzene rings (A- and B-rings) having a phenolic hydroxyl group are linked by a central three-carbon atom, and the basic mother nucleus is 2-benzene. Primary ketone. A functional group such as a phenolic hydroxyl group, a methoxy group, a methyl group or an isopentenyl group is often bonded to the flavonoid structure. In addition, it often combines with sugars to form glycosides. OBJECTIVE: To investigate the effects of extraction solvent, extraction time and extraction times on the yield of total flavonoids in Radix Astragali. Methods: UV spectrophotometry was used to determine the total flavonoid content obtained by different extraction solvents, different extraction times and different extraction times. RESULTS: The optimal extraction process of total flavonoids from Astragalus membranaceus was found by orthogonal experiment. Conditions: 30% ethanol extraction 3 times, dosage: 12, 10, 10 times, time: 2, 1, 1h. Conclusion: This study can provide a theoretical basis for the development of a reasonable extraction process for the production of total flavonoids from Astragalus membranaceus, which has certain guiding significance for production. Scutellariabaicalensis Georgi is one of the commonly used traditional Chinese medicines. It has the effects of clearing away heat and dampness, purging fire and detoxification, and stopping bleeding and fetus. Its main active ingredient is flavonoids. Modern pharmacological studies have proved that Astragalus membranaceus not only has obvious antibacterial, anti-inflammatory, anti-allergic, anti-oxidant, anti-cancer and anti-viral effects, but also has a certain effect on cardiovascular diseases. Most of the research reports on the extraction of baicalin from Astragalus membranaceus are decoctioned with water as a solvent, but the yield of baicalin is low and the production cycle is long. In this study, orthogonal experiments were carried out, and total flavonoids were used as indicators. The influencing factors were studied to obtain a better extraction process, which greatly improved the yield. 1 Materials and instruments 1.1 Materials Medicinal materials: Astragalus; Reagents: Baicalin standard; Methanol; Methanol; Phosphoric acid. 1.2 Instrument:UV-1500PC ultraviolet spectrophotometer
(American Analysis (China) Instrument Co., Ltd.). 2 Methods and Results 2.1 Preparation of the standard curve of total flavonoids of Astragalus membranaceus accurately weighed 3 mg of the dried to constant weight baicalin standard, fixed to 100 ml volumetric flask with methanol, shake well, used as a reference solution, and set aside. A UV scan of 190-500 nm was performed using a reference solution using methanol as a blank control. The scan results showed a maximum absorption at a wavelength of 277.6 nm, so 277.6 nm was chosen as the detection wavelength. When the stability of the reference solution was observed within 1 h, the change was small, so the total flavonoids of Astragalus membranaceus were directly determined without the use of a color developer. Accurately draw reference solution 0.00, 1.00, 2.00, 3.00, 4.00, 5.00ml, separate into a 10ml volumetric flask, dilute to volume with methanol, shake well, use 0.00ml of reference solution as blank, measure absorbance at 277.6nm (A). Linear regression was performed on the concentration (C) of A, and the equation was Y = 66.878X-0.003 (R2 = 0.9996, r = 0.9998). 2.2 Orthogonal design optimization of the extraction process of total flavonoids from Astragalus membranaceus L9 (34) orthogonal table to select the best extraction process of Astragalus. Weigh 9 parts of the medicine, 50g each. Extracted with 30% ethanol, taking the total flavonoids as an indicator, the three factors affecting the extraction effect were investigated: solvent dosage, optimal extraction time, and extraction times. Each medicinal material was extracted and filtered, and the filtrate was combined. The absorbance (A) was measured at 277.6 nm, and the total flavonoid content in the extract was determined. 3 Results analysis From the results of orthogonal analysis and analysis of variance, the effect of various factors on the total flavonoids content of Astragalus membranaceus was C>A>B, and C was a very significant difference factor, A and B were significant differences. , C3>C2>C1, A1>A2>A3, B3>B2>B1, the best extraction condition is A1B3C3, ie solvent dosage (12,10,10), extraction time (2,1,1)h, extraction 3 The second is the best extraction process conditions. 4 Discussion The commonly used methods for the determination of flavonoids by spectrophotometry include direct determination, Al(NO3)3 and AlCl3. In order to select a suitable method, we used a reference solution with methanol as a blank to carry out UV at 190-500 nm. scanning. The scanning results showed that the maximum absorption value at the wavelength of 277.6 nm and the peak shape were good, so 277.6 nm was selected as the detection wavelength. Further observation of the stability of the reference solution in 1 h, the change is very small, so the direct determination of total flavonoids of Astragalus membranaceus was carried out without the use of a color developer. According to the experimental results, the optimal process for this experiment is as follows: the extraction solvent is 30% ethanol, and the extraction is carried out 3 times, respectively, adding 12, 10, 10 times the solvent, and the extraction time is 2, 1, 1 h. In order to verify this result, take 3 herbs, 50g each, according to the selected extraction process: extract solvent 30% ethanol, extract 3 times, add 12, 10, 10 times solvent, extraction time 2, 1, 1h. Finally, the total flavonoid content was 19.03%, 18.65%, and 19.48%. Through the verification experiment, the extraction efficiency of total flavonoids was better. Explain that the selected process is stable and effective and is suitable for continued amplification. Key words: astragalus; flavonoids; extraction; ultraviolet spectrophotometry;Aesthetic instrument
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