Biosensors meet people's requirements. At present, quite a few biosensors have been used in environmental monitoring. In water monitoring and operation control of sewage treatment plants, biochemical oxygen demand is also one of the most commonly used and important indicators.
In recent years, the problem of environmental pollution has become increasingly serious, and serious environmental pollution has also caused a great impact on people's lives. Many diseases are caused by environmental factors. The monitoring of environmental pollution cannot be ignored. Now biosensors meet people's requirements, and a considerable number of biosensors have been used in environmental monitoring.
â‘´Water environment monitoring
Biochemical Oxygen Demand (BOD) is a widely used comprehensive indicator to characterize the degree of organic pollution. In water body monitoring and operation control of sewage treatment plants, biochemical oxygen demand is also one of the most commonly used and important indicators. The routine BOD measurement requires a 5d incubation period, and the operation is complicated, the repeatability is poor, time-consuming and laborious, and the interference is large, which is not suitable for on-site monitoring. SiyaWakin et al. Used a Trichosporoncutaneum and Bacilluslicheniformis to make a microbial BOD sensor. The BOD biosensor can accurately measure the concentration of glucose and glutamic acid at the same time. The measurement range is 0.5-40mg / L, and the sensitivity is 5.84nA / mgL. The biosensor has good stability. In 58 experiments, the standard deviation is only 0.0362. The required reaction time is 5-lOmin.
NO3 ion is one of the main water pollutants. If added to food, it is extremely harmful to human health. Zatsll et al. Proposed an integrated enzyme function field effect tube device to detect NO; ion method. The detection limit of the device for NO and ions is 7x10 minus 5 mol, the response time is less than 50s, and the system operation time is about 85s. In addition, there are reports that Han et al. Fixed Pseudomonas on the ion-grabbing electrode , Real-time monitoring of trichloroethylene in industrial wastewater, the detection range is 0.1 to 4mg / L, and the detection time is within 10min.
⑵Atmospheric environment monitoring
Sulfur dioxide (S02) is the main cause of acid rain and acid mist formation, and the traditional detection method is very complicated. Martyr et al. Fixed subcellular lipids (liver microsomes containing sulfite oxidase) on cellulose acetate membrane, and made an amperometric biosensor with oxygen electrode to detect the acid rain and acid mist sample solution formed by S02. lOmin can get stable test results.
NOx is not only one of the causes of acid rain and acid mist, but also the culprit of photochemical smog. Charles et al. Used a microbial sensor composed of a porous permeable membrane, immobilized nitrifying bacteria and an oxygen electrode to determine the content of nitrite in the sample, thereby inferring the concentration of NO = in the air. The detection limit is 0.01xl0 min 6th power mo1 / L.
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